EGCG pretreatment enhanced adaptive autophagy and alleviated Dox-induced myocardial ferroptosis in mice. A Diagram of the in vivo study design. B CK-MB and LDH activity in mouse serum in each treatment group. C The iron contents of mouse serum and myocardium in each treatment group. D Echocardiographic images, and the histogram of LVEDD, LVESD, LVEF, and LVFS parameters in mice. Values are mean ± SD from six individual experiments. **p < 0.01 compared with the indicated groups. E Images of HE-stained mouse myocardium. F Images of Masson-stained mouse myocardium. G Images of TUNEL-stained mouse myocardium. H Transmission electron microscope images and the relative Fiameng scores of mouse myocardium. I Western blot bands showing PTGS2, GPX4, LC3, and P62 protein expression in mouse myocardium. J Relative intensities of PTGS2 and GPX4 expression in mouse myocardium. K Relative intensities of LC3 and P62 expression in mouse myocardium. Values are mean ± SD from three individual experiments. NS, nonsignificant. **p < 0.01 compared with the indicated groups.