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Expression of Bop1Δ causes a deficit in 60S ribosomal subunits. The inducible Bop1Δ expression cell line, line 6, was grown in the absence (A) or presence (B) of IPTG as indicated for 38 h. Cytoplasmic extracts were isolated and separated on a 10 to 45% sucrose density gradient. Profiles of absorbance at 254 nm (A254) profiles revealed the positions and relative amounts of the ribosomal subunits in the gradient.
