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. 2021 Nov 4;15:734158. doi: 10.3389/fnins.2021.734158

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Copyright © 2021 de Paula, Brunetta, Engel, Gaspar, Velloso, Engblom, de Oliveira and de Bem.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hippocampal alterations followed by 4 weeks of HFD. (A) Representative experiment in SUIT protocol. (B) Respirometric measurements by oxygen flux using PM as initial substrates (leak), followed by ADP (CIp), S (Oxphos), FCCP titrations (ETS), and finally inhibited by Rot (CIIp; F = 2.077) and Ant A in the hippocampus of mice submitted to 4 weeks of SD or HFD (n = 5–6). (C) Representative images of GFAP immunolabeling in the hippocampus of 4 weeks SD and HFD-fed mice (scale bar = 150 μm) along with (D) quantification of GFAP immunoreactivity (n = 4). Values are mean ± SEM. Unpaired t-test performed. *p < 0.05, **p < 0.01 SD vs. HFD.