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. 2021 Nov 4;12:758613. doi: 10.3389/fmicb.2021.758613

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Copyright © 2021 Elizalde, Tadey, Mammana, Quarleri, Campos and Flichman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of cccDNA levels among genotypes. HuH-7 cells were transfected with linear full-length HBV genomes of genotypes A2, B2, C1, D1, and F1b in the presence or in the absence of 100μm Lamivudine. 3days post-transfection, cells were harvested, total DNA was extracted, and cccDNA levels were assessed by qPCR. Results were normalized to mitochondrial DNA. Shown values represent the mean±standard deviation of three independent experiments. ^*: statistical difference in relation to gtA2, ξ: difference in relation to gtB2, #: difference in relation to gtC1 and &: difference in relation to gtD1. One symbol: p<0.005 and two symbols: p<0.0001.