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. 2021 Nov 4;12:758613. doi: 10.3389/fmicb.2021.758613

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Copyright © 2021 Elizalde, Tadey, Mammana, Quarleri, Campos and Flichman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of HBV RNA levels across genotypes. HuH-7 cells were transfected with linear full-length HBV genomes of genotypes A2, B2, C1, D1, and F1b. Three days post-transfection, cells were harvested, total RNA was extracted, and pgRNA (A) and Precore mRNA (B) levels were analyzed by transcript-specific RT-qPCR. (C) cccDNA replicative activity was determined as molecules of pgRNA synthesized per cccDNA (pgRNA/cccDNA). (D) The number of Precore mRNA transcripts produced per cccDNA (Precore RNA/HBVccc) was used to assess HBV transcriptional efficiency. Values shown represent the mean±standard deviation of three independent experiments. ^*: difference in relation to gtA2, ξ: difference in relation to gtB2, #: difference in relation to gtC1 and &: difference in relation to gtD1. Two symbols: p<0.0001.