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. 2021 Nov 4;12:758613. doi: 10.3389/fmicb.2021.758613

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Copyright © 2021 Elizalde, Tadey, Mammana, Quarleri, Campos and Flichman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of intracellular and secreted HBsAg levels across genotypes. HuH-7 cells were transfected with linear full-length HBV genomes of genotypes A2, B2, C1, D1, and F1b. Three days post-transfection, cells and culture supernatants were harvested, and intracellular and extracellular levels of HBsAg were determined by electrochemiluminescence immunoassay (A). Extracellular/intracellular HbsAg ratio (B). Values shown represent the mean±standard deviation of three independent experiments. ^*: Intracellular or extracellular difference in relation to genotype A2, ξ: Intracellular or extracellular difference in relation to genotype B2, #: Intracellular or extracellular difference in relation to genotype gtC1 and &: Intracellular or extracellular difference in relation to genotype D1. Two symbols: p<0.0001.