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. 2021 Nov 4;12:758613. doi: 10.3389/fmicb.2021.758613

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Copyright © 2021 Elizalde, Tadey, Mammana, Quarleri, Campos and Flichman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative composition of HBsAg proteins among genotypes. HuH-7 cells were transfected with linear full-length HBV genomes of genotypes A2, B2, C1, D1, and F1b. Three days post-transfection, cellular lysates (A) and the corresponding culture supernatants (B) were analyzed by Western Blot, using a SHBs specific monoclonal (HB01) antibody. As this antibody shows a decreased reactivity for HBsAg derived from genotype F transfected cells, an increased time of exposure was needed. SHBs and LHBs occur in unglycosylated (LHBs and SHBs) and glycosylated (gLHBs and gSHBs) forms, while MHBs occurs in glycosylated (gMHBs) and double glycosylated (ggMHBs) forms (C) Intracellular and (D) extracellular LHBs/MHBs/SHBs ratio was determined. Ct: cell transfected with pUC19 empty vector (control).