Figure 6.

Analysis of intracellular and secreted HBeAg levels across genotypes. HuH-7 cells were transfected with linear full-length HBV genomes of genotypes A2, B2, C1, D1, and F1b. Three days post-transfection, cells and culture supernatants were harvested, and intra- and extracellular levels of HBeAg were determined by ECLIA (A). Extracellular/intracellular HBeAg ratio (B). Shown values represent the mean±standard deviation of three independent experiments. ^*: Intracellular or extracellular difference in relation to genotype A2, ξ: Intracellular or extracellular difference in relation to genotype B2, and #: Intracellular or extracellular difference in relation to genotype gtC1. One symbol: p<0.005 and two symbols: p<0.0001.