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. 2021 Nov 4;15:752594. doi: 10.3389/fnins.2021.752594

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Copyright © 2021 Sanchez-Varo, Sanchez-Mejias, Fernandez-Valenzuela, De Castro, Mejias-Ortega, Gomez-Arboledas, Jimenez, Sanchez-Mico, Trujillo-Estrada, Moreno-Gonzalez, Baglietto-Vargas, Vizuete, Davila, Vitorica and Gutierrez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hippocampal soluble fractions from young APP/PS1 mice reduce the synaptic protein content in vitro. Primary neuronal cultures were incubated with soluble fractions (S1) from 6-month-old APP/PS1 or WT mice for 48 h (n = 3/group). Incubation with PBS was used as an additional control. Analysis by Western blot (A) and quantification showed a significant decrease in the protein levels of the presynaptic markers VGLUT1 (B) [ANOVA F_{(2, 9)} = 4.38 P = 0.05; Tukey’s *p < 0.5] and SYN (C) [ANOVA F_{(2, 9)} = 8.03 P = 0.05; Tukey’s *p < 0.05] after incubating with APP/PS1 S1, but not when incubating with control fractions. As a proof of the selective synaptic damage, NeuN protein levels showed no significant changes (D). Represented data were normalized respect to PBS.