Skip to main content
. 2021 Nov 4;14:748214. doi: 10.3389/fnmol.2021.748214

FIGURE 5.

FIGURE 5

Dual functions of phosphodiesterases in intensity-dependent olfactory encoding. (A–F) 0.01% IAA-induced currents from AWC neurons in pde–1(nj57);–2(nj58);–3(nj59);–5(nj49) quadruple mutants. (A) Representative trace. (B) The amplitude of olfactory currents was normal in pde quadruple mutants. The rise time of both outward currents (C) and inward currents (D) become slower, and the decay time of inward currents induced by odorant removal become much longer (E). (F) Input resistance of AWC neurons was decreased in pde quadruple mutants. n ≥ 6. (G–L) 0.01 and 1% IAA-induced currents from AWB neurons in pde–1(nj57);–2(nj58);–3(nj59);–5(nj49) quadruple mutants. (G) Representative traces. (H) Quantification of the currents. 0.01% IAA-induced currents from AWB neurons were abolished in pde quadruple mutants. The amplitudes of 1% IAA-induced outward currents were increased and inward currents were decreased in pde quadruple mutants. The rise times of both 1% IAA-induced outward currents (I) and inward currents (J) become much slower, and the decay time of inward currents induced by 1% IAA removal become much longer (K). Input resistance of AWB neurons was decreased in pde quadruple mutants (L). n ≥ 6. (M,N) 0.01% IAA-induced calcium increases in AWB neurons were abolished in pde quadruple mutants. (M) Representative traces. (N) Quantification of the maximumΔF/F0. n ≥ 12. (O,P) 1% IAA-induced calcium decreases in AWB neurons were retained in pde quadruple mutants. (O) Representative traces. (P) Quantification of the maximumΔF/F0. n ≥ 6. (Q,R) A puff of either 1 mM IBMX or 0.01% IAA induced an inward current from AWB neurons. (Q) Representative traces. (R) Quantification of the currents. Black square: IBMX. Red square: 0.01% IAA. A puff of 0.5% DMSO was taken as negative control. No summation of the currents induced by IBMX and 0.01% IAA was observed, when a puff of IBMX was followed by a successive puff of 0.01% IAA within 500ms interval. n ≥ 3. Error bars: SEM. N.S., not significant, *P < 0.05, **P < 0.01, ***P < 0.001.