Reagent or Resource	Source	Identifier
Chemicals, Peptides, and Recombinant Proteins
2, 3, 5-Trimethylthiazole	Sigma-Aldrich	Cat#W332518
Benzaldehyde	Sigma-Aldrich	Cat#B1334
Isoamyl alcohol	Sigma-Aldrich	Cat#W205702
Ethyl alcohol	Sigma-Aldrich	Cat#459836
2,3-pentanedione	Sigma-Aldrich	Cat#A5835
LY83583	Sigma-Aldrich	Cat#A6563
3-isobutyl-1-methyl-xanthine	Sigma-Aldrich	Cat#I5879
Critical Commercial Assays
In-Fusion HD Cloning Kit	Takara	Cat#639649
Experimental Models: Organisms/Strains
N 2	CGC	ST348
oyIs44[odr-1:RFP]	CGC	PY2417
tax-4(ks11);oyIs44[Podr-1:RFP]	This study	ST426
tax-2(P691),oyIs44[Podr-1:RFP]	This study	ST481
unc-13(e1091),oyIs44[Podr-1:RFP]	This study	ST483
unc-13(e51),oyIs44[Podr-1:RFP]	This study	ST486
kanEx020[Pstr-2:GFP];oyIs44[Podr-1:RFP]	This study	ST523
unc-31(e928);oyIs44[Podr-1:RFP]	This study	ST576
unc18(e81);oyIs44[Podr-1:RFP]	This study	ST497
N2;kanEx22[Pstr-2:GFP]	This study	ST490
pde-1(nj57);pde-2(nj58);pde-3(nj59);pde-5(nj49);kanEx22[Pstr-2:GFP]	This study	ST600
pde-4(nj60),pde-6(ok2410);kanEx22[Pstr-2:GFP]	This study	ST595
odr-3(n2150);kanEx22[Pstr-2:GFP]	This study	ST601
gpa-3(pk35); kanEx22 [Pstr-2:GFP]	This study	ST909
odr-1(n1936); oyIs44[Podr-1:RFP]	This study	ST540
nt1/daf-11(m47);oyIs44[Podr-1:RFP]	This study	ST541
gpa-3(pk35); odr-3(n1605);kanEx22 [Pstr-2:GFP]	This study	ST645
kanEx41[Podr-1:DsRed2b]	This study	ST656
kanEx23[Psra-6:Dsred + Pstr-3:yfp]	This study	ST717
osm-9(ky10);kanEx23[Psra-6:Dsred + Pstr-3:yfp]	This study	ST718
odr-3(n2150);kanEx23[Psra-6:Dsred + Pstr-3:yfp]	This study	ST550
cng-1(jh111); cng-3(jh113);kanEx41[Podr-1:Dsred2b]	This study	ST637
odr-3(n2150); kanEx41[Podr-1:DsRed2b]	This study	ST772
kanEx81[Pstr-1:Dsred]	This study	ST793
pde-1(nj57);pde-2(nj58);pde-3(nj59);pde-5(nj49); kanEx81[Pstr-1:Dsred]	This study	ST794
odr-3(n2150); kanEx81[Pstr-1:Dsred]	This study	ST834
gpa-3(pk35);odr-3(n1605); kanEx81[Pstr-1:Dsred]	This study	ST838
gpa-3(pk35); kanEx81[Pstr-1:Dsred]	This study	ST906
kanEx266[Pstr-1:mcherry + Pstr-1:GCaMP5.0 + Plin-44:gfp]	This study	ST1890
pde-1(nj57); kanEx266[Pstr-1:mcherry + Pstr-1:GCaMP5.0 + Plin-44:gfp]	This study	ST1740
pde-2(nj58); kanEx266[Pstr-1:mcherry + Pstr-1:GCaMP5.0 + Plin-44:gfp]	This study	ST1736
pde-3(nj59); kanEx266[Pstr-1:mcherry + Pstr-1:GCaMP5.0 + Plin-44:gfp]	This study	ST1738
pde-5(nj49); kanEx266[Pstr-1:mcherry + Pstr-1:GCaMP5.0 + Plin-44:gfp]	This study	ST1742
pde-1(nj57);pde-2(nj58);pde-3(nj59);pde-5(nj49); kanEx266[Pstr-1:mcherry + Pstr-1:GCaMP5.0 + Plin-44:gfp]	This study	ST1313
odr-3(n2150); kanEx266[Pstr-1:mcherry + Pstr-1:GCaMP5.0 + Plin-44:gfp]	This study	ST1310
gpa-3(pk35); kanEx266[Pstr-1:mcherry + Pstr-1:GCaMP5.0 + Plin-44:gfp]	This study	ST1311
kanEx277[Psrh-220:mcherry + Plin-44:gfp]	This study	ST1320
unc-13(e51); kanEx266[Pstr-1:mcherry + Pstr-1:GCaMP5.0 + Plin-44:gfp]	This study	ST1427
unc-31(e1091);kanEx266[Pstr-1:mcherry + Pstr-1:GCaMP5.0 + Plin-44:gfp]	This study	ST1428
gpa-3(pk35);kanEx480[Pstr-1:gpa-3 cDNA:sl2a-tagRFP + Pstr-1:GCaMP5.0 + Plin-44:gfp]	This study	ST1700
kanEx52[Podr-10:mCherry + Punc-122:gfp]		This study	ST568
osm-9(ky10);kanEx52[Podr-10:mCherry + Punc-122:gfp]		This study	ST569
Software and Algorithms
ImageJ	NIH	https://imagej.nih.gov/ij/; RRID:SCR_003070
Micro-Manager	Vale Lab, UCSF	http://micro-manager.org, RRID:SCR_000415.