Fig. 3.

¹³C time-resolved spectrum of ¹³C-1-enriched polyM treated with AlyA3. (A) Pseudo 2D time-resolved spectra recorded by acquiring 1D carbon spectra every 5 min for a total of 16 h 40 min. Signals associated with C-1 of each component are labeled. (B) Normalized integrals of monomeric products produced during treatment of polyM with AlyA3. (C) Structures of the three main products. Δ: 4,5-unsaturated 4-deoxy-l-erythro-hex-4-enepyranosyluronate; DHF: 4-deoxy-d-manno-hexulofuranosidonate. Reaction was conducted in 3-mm NMR tube with 160 μL of substrate buffer solution (10 mg mL^{−1 13}C-1-polyM substrate in 10-mM HEPES, pH 7.0 with 2-mM NaCl in 99.9% D₂O) and 10 μL of 0.9-μM AlyA3 incubated at 25°C. This figure is available in black and white in print and in color at Glycobiology online.