Table III.
Kinetic parameters of AlyA3 on different substrates
| Substrate | K m (μM) | Vm (μM min−1) | k cat (s−1) | k cat/Km (mM−1 s−1) |
|---|---|---|---|---|
| Alginate M/G = 2 | 4355 ± 4158 | 1.7 ± 1.0 | 4.7 ± 2.9 | 1.1 ± 0.9 |
| M-blocks | 1250 ± 520 | 14.2 ± 2.6 | 39.5 ± 7.2 | 31.6 ± 8.2 |
| M-DP2 | 603 ± 208 | 4.8 ± 0.6 | 13.4 ± 1.6 | 22.2 ± 3.7 |
| M-DP3 | 781 ± 245 | 16.0 ± 1.9 | 44.5 ± 5.3 | 57.0 ± 9.5 |
| M-DP4 | 786 ± 221 | 13.8 ± 1.5 | 38.3 ± 4.1 | 48.8 ± 7.3 |
| M-DP5 | 414 ± 172 | 13.9 ± 1.8 | 38.6 ± 4.9 | 93.1 ± 16.8 |
| M-DP6 | 428 ± 199 | 15.5 ± 2.4 | 43.1 ± 6.6 | 100.4 ± 21.9 |
Experiments were performed at 35°C, in a Tris–HCl buffer (100 mM, pH 7.0). Substrate concentration range was 0.025–0.5 g L−1. Values are derived from independent triplicate experiments.