Table 2.

Primer sequences and genetic characteristics of the loci used for MLST.

Locus	Forward primer	Reverse primer	Amplified fragment (pb)	Coding region	Number of alleles	Number of polymorphic sites	H (n=89)a	dN-dSb
dnaN	GCACATGTTAATTCGCCAGAGG	CAGCACCAACTCTGATAATTTTCCA	404	[1–237][299–404]	4	4	0,3,164	−0,547
mutL	CCAACCAAGCAGGAAGTTCG	CGTTCTTGAGCTGCGTGTTG	545	[1–503]	5	9	0,2,814	−0,77
mutM	CAGAGTAGATGGTTTGACCC	TGCCCTGTATGATGCCTATC	410	[1–157]	4	5	0,2061	−0,358
mutS	TTTAACTGGCGCATCCCCAT	TGGATCTTGCAACAGGTGAGT	448	[1–448]	4	15	0,3,639	0,995
mutX	TGGCCATTGGTTTCATCAAGG	CGTAATCCCCTTCCCACGTT	547	[172–547]	3	4	0,0876	−1,553
recD2	AGGGCTTCCTAGTGCTACCA	ACTCGCTTTGCCCATCAAGA	563	[1–563]	5	6	0,2,658	−1,594
rnhC	GGAATCGCTGTTGTT GCAAGT	TTGAGTGTTTGCGAAGTGGC	582	[1–582]	3	4	0,0447	1,768
yfhQ	AGGCCAGGTGATTTCAACCA	CAGGAGAAACCCAGGCCATT	511	[1–511]	3	6	0,2040	−1,089

^agenetic diversity expressed as the probability that, at a single locus, any two alleles, chosen at random from the population, are different to each other.

^bnon-synonymous (d_N)–synonymous substitutions (d_S).