Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 18;14:417. doi: 10.1186/s13104-021-05831-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — UDP-Glo assay optimization with sialic acid trimer (DP3). A Assay results with different NmW enzyme amounts with maximum activity observed with 1250 ng enzyme. B Assay results using different concentrations of DP3 acceptor indicate maximum activity with 4 mM DP3. C Results with different concentrations of CMP-NeuNAc and UDP-Gal show more activity in the presence of 80 µM of both donor sugars (Mean = 1,01,833 RLU) vs 100 µM (98,566.7). For panel A, the data point indicates the mean and error bars represent standard deviation. For panels B and C, top of bar graph indicates the mean and error bars represent standard deviation. Each panel illustrates representative examples of three individual experiments. All experiments were run with three replicates