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. 2021 Nov 4;12:735530. doi: 10.3389/fphar.2021.735530

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Copyright © 2021 Jiang, Dong, Zhai, Lou, Jin, Cheng, Chen, Guo, Lin, Ding and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Paeoniflorin (PF) protected HUVECs from TBHP-induced oxidative stress via HO-1 activation. (A) Evaluation of total HO-1 protein by Western blotting. (B) Percentage of DCFDA staining intensity under different treated groups and detected by fluorescence microscope. (C) Representative images of DCFDA fluorescence performed to exhibit the intracellular ROS level under different conditions. (D,E) Evaluation of HO-1, cleaved caspase-3, Bcl-2, and Bax proteins using Western blotting for each group treated with/without TBHP, PF, and protoporphyrin IX (SnPP). Data: mean ± SD, **p < 0.01 vs controls. #p < 0.05, ##p < 0.01 vs. TBHP-stimulated HUVECs. @p < 0.05, @@p < 0.01 vs. TBHP+PF co-treated HUVECs. n = 3.