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. 2021 Nov 8;47(1):10. doi: 10.3892/or.2021.8221

Figure 2.

Figure 2.

Global chromatin methylation, gene-specific quantitative methylation and gene expression after 5AZA and decitabine treatment. (A) Chromatin methylation after 48 h or 7 days from a single administration of the indicated drug. ***P<0.0001 (n=3; one-way ANOVA). (B) Quantitative promoter methylation of KLF4, DAPK1 and SPG20 after the administration of either 1 µM 5AZA or decitabine 0.5 µM for 48 h. (C) Gene expression of the selected targets upon treatment with hypomethylating drugs (cutoff, 37 cycles). n=2/3. (D) KLF4, DAPK1 and SPG20 protein expression levels after treatment with hypomethylating drugs for 48 h. n=3. The blots of GNE-587170 protein extracts are presented as positive controls. NT, not treated; KLF4, Krüppel-like factor 4; DAPK1, death associated protein 1; SPG20, spastic paraplegia 20; 5AZA, 5′-aza-cytidine; decitabine, 5′-aza-2-deoxycitidine.