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. 2021 Nov 8;47(1):10. doi: 10.3892/or.2021.8221

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Copyright: © Frazzi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Genome-wide methylation analysis by Infinium Human Methylation 450K BeadChip array and MS-PCR. (A) Heatmap of the β-values of SPG20 locus in 28 diffuse large B cell lymphoma cell lines. (B) Bioinformatics localization of the 35 probes identifying SPG20 locus. (C) MS-PCR on cg15754752 probe with M- or U-specific primers. (D) β-value distribution of the nine probes mapping upstream or on the SPG20 TSS. M, methylated; U, unmethylated; MS-PCR, methylation-specific PCR; SPG20, spastic paraplegia 20; TSS, transcription starting site; DNMT1, DNA methyltransferase 1; NT, not treated; si, small interfering RNA.