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. 2021 Nov 2;25(1):3. doi: 10.3892/mmr.2021.12519

Figure 2.

Figure 2.

Overexpression of MEG3 alleviates TGF-β1-stimulated hepatic stellate cell activation. (A) Following overexpression vector transfection, MEG3 expression levels in LX-2 cells were detected by RT-qPCR. (B) Following MEG3 overexpression and TGF-β1 treatment, MEG3 expression levels in LX-2 cells were detected by RT-qPCR. (C) LX-2 cell proliferation was quantified using the Cell Counting Kit-8 assay. ##P<0.01 vs. control, **P<0.01 vs. TGF-β1+vector. (D) Immunofluorescence was used to determine α-SMA and COL1A1 expression in LX-2 cells. Representative images from each group are presented (magnification, ×100). (E) Protein expression levels of α-SMA and COL1A1 in LX-2 cells were detected by western blotting. **P<0.01 vs. Control; #P<0.05 vs. TGF-β1+vector. α-SMA, α-smooth muscle actin; COL1A1, collagen type 1 α1; MEG3, maternally expressed gene 3; RT-qPCR, reverse transcription-quantitative PCR.