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. 2021 Nov 2;25(1):3. doi: 10.3892/mmr.2021.12519

Figure 5.

Figure 5.

miR-145 negatively regulates PPARγ in LX-2 cells. (A) Predicted miR-145 target sites on PPARγ 3′untranslated region. (B) Dual-luciferase reporter assays were performed to verify the relationship between miR-145 and PPARγ. **P<0.01. PPARγ (C) protein and (D) mRNA expression levels in serum from healthy patients and patients with mild and severe fibrosis were detected by western blotting and reverse transcription-quantitative PCR, respectively. **P<0.01; #P<0.05. (E) Verification of miR-145 mimic and inhibitor transfection efficiency. **P<0.01 vs. NC-mimic; ##P<0.01 vs. NC-inhibitor. (F) Following transfection with miR-145 mimic or inhibitor, PPARγ protein expression levels in LX-2 cells were detected by western blotting. **P<0.01 vs. NC-mimic; ##P<0.01 vs. NC-inhibitor. miR, microRNA; MUT, mutant; NC, negative control; PPARγ, peroxisome proliferator-activated receptor γ; WT, wild-type.