Table II.
Gene | Primer sequence (5′→3′) |
---|---|
PPARγ | F: ATTTTCTGGAGAGCTTGGC |
R: GTGAGGGTCTCTCTCTTCCT | |
α-SMA | F: GACGAAGCACAGAGCAAAAG |
R: ACAGCACCGCCTGGATAG | |
COL1A1 | F: GAGGCATGTCTGGTTCGG |
R: TGGTAGGTGATGTTCTGGGAG | |
MEG3 | F: CCTGCTGCCCATCTACACCTC |
R: CCTCTTCATCCTTTGCCATCCTGG | |
miR-145 | F: ATATCTCGAGGGAGAGAGATGCCTTCAGCA |
R: ATTTATAAGCTTGGAATCCTTCTCAACACTGAATATCTAC | |
U6 | F: CTCGCTTCGGCAGCACA |
R: AACGCTTCACGAATTTGCGT | |
GAPDH | F: AATCCCATCACCATCTTC |
R: AGGCTGTTGTCATACTTC |
PPARγ, peroxisome proliferator-activated receptor γ; α-SMA, α-smooth muscle actin; COL1A1, collagen type 1 α1; F, forward; MEG3, maternally expressed gene 3; miR, microRNA; R, reverse.