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. 2021 Nov 10;47(1):12. doi: 10.3892/or.2021.8223

Figure 2.

Figure 2.

miR-190b suppresses AsPC-1 cell invasion in vitro. (A) AsPC-1 cells were transfected with miR-190b mimics or NC. Following transfection with miR-190b mimics, miR-190b expression increased by 42.88±2.18-fold. (B) AsPC-1 cells were transfected with anti-miR-190b or anti-NC. Following transfection with anti-miR-190b, miR-190b expression decreased by 0.30±0.03-fold. (C) Representative photomicrographs of Transwell assay results for AsPC-1 cells (original magnification, ×100). (D) The numbers of miR-190b-transfected AsPC-1 cells invading through the Matrigel membrane were significantly lower compared with NC-transfected and parental AsPC-1 cells (blank control). Cells were counted in 16 independent symmetrical visual fields under an inverted microscope (original magnification, ×400) in three independent experiments. (E) The numbers of anti-miR-190b-transfected AsPC-1 cells invading through the Matrigel membrane were significantly higher compared with anti-NC-transfected and parental AsPC-1 cells (blank control). Cells were counted in 16 independent symmetrical visual fields under an inverted microscope (original magnification, ×400) in three independent experiments. (F) Representative photomicrographs of Transwell assay results for AsPC-1 cells (original magnification, ×100). Values are shown as mean ± SD; n=3. *P<0.05. miR, microRNA; NC, negative control.