Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 10;47(1):12. doi: 10.3892/or.2021.8223

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 3. — miR-190b suppresses MIA PaCa-2 cell invasion in vitro. (A) MIA PaCa2 cells were transfected with miR-190b mimics or NC. Following transfection with miR-190b mimics, miR-190b expression increased by 354.62±69.44-fold. (B) MIA PaCa-2 cells were transfected with anti-miR-190b or anti-NC. Following transfection with anti-miR-190b, miR-190b expression decreased by 0.34±0.05-fold. (C) Representative photomicrographs of Transwell assay results for MIA PaCa-2 cells (original magnification, ×100). (D) The numbers of miR-190b-transfected MIA PaCa-2 cells invading through the Matrigel membrane were significantly lower compared with NC-transfected and parental MIA PaCa-2 cells (blank control). Cells were counted in 16 independent symmetrical visual fields under a light microscope (original magnification, ×400) in three independent experiments. (E) The numbers of anti-miR-190b-transfected MIA PaCa-2 cells invading through the Matrigel membrane were significantly higher compared with anti-NC-transfected and parental MIA PaCa-2 cells (blank control). Cells were counted in 16 independent symmetrical visual fields under a light microscope (original magnification, ×400) in three independent experiments. (F) Representative photomicrographs of Transwell assay results for MIA PaCa-2 cells (original magnification, ×100). Values are shown as mean ± SD; n=3. *P<0.05. miR, microRNA; NC, negative control.