Figure 5.

miR-190b suppresses pancreatic cancer growth and metastasis in vivo. (A) Xenograft mouse models were used to detect the proliferative ability of AsPC-1 cells in vivo. Images of tumors in each group up to day 16 after injection. (B) Mean tumor weight in each group. (C) Tumor growth curves based on in vivo tumor volume. (D) Representative microscopic images showing histological morphology (H&E staining; magnification, ×100) and Ki67 expression [magnification, ×100 and ×400 (inset)]. Scale bar, 100 µm. (E) Representative images of mouse lung tissues (upper panels) and corresponding histological sections (lower panels). Cell aggregates with dark-stained nuclei represent lung metastases (arrows). (F) Data are shown graphically by the number of lung metastases at 4 weeks in each mouse injected with 1×10⁶ AsPC-1 cells. Values are shown as mean ± SD; n=8. *P<0.05. miR, microRNA; NC, negative control.