Table II.
Primers and DNA segments used for vector construction.
| Genes | Sequences (5–3) |
|---|---|
| MEF2C | F: GCTCTAGATGCAGTTGCACATTTGAG |
| R: GCTCTAGAAATGGTGAGATCAGAAAGG | |
| TCF4 | F: GCTCTAGAAAACAAGAGACCACTTCC |
| R: GCTCTAGAAGTTTAGGCACAAATGC | |
| MEF2C-MUTa | GCTCTAGATGCAGTTGCACATTTGAGTAACTATTTTCTTTCTGTTTTCTT TTACTCTGCTTACATTTT |
| ATAAGTTTAAGGTCAGCTGTCAAAAGGATAACCTGTGG GGTTAGACTGAGGACCATTGCAACACC | |
| CTAAATTGTTTTTAATACATTAGCAATCTAT TGGGTCAACTGACATCCATTGTATATACTAGTTTCTT | |
| TCATGCTATTTTTATTT TGTTTTTTGCATTTTTATCAAATGCAGGGCCCCTTTCTGATCTCACCATTT | |
| CTAGAGC | |
| TCF4-MUT | GCTCTAGAAAACAAGAGACCACTTCCTTAACAGCTGTATTATCTTAAACCCACAT AAACACTTCTC |
| CTTAACCCCCATTTTTGTAATATAAGACAAGTCTGAGTAGTTATGAA TCGCAGACGCAAGAGGTTT | |
| CAGCATTCCCAATTATCAAAAAACAGAAAA ACAAAAAAAAGAAAGAAAAAAGTGCAACTTGAG | |
| GGACGACTTTCTTTACGTCCGATTTCAGAATGTGCAAAGCAGTATGTACAGGCTGAGACACAGCC | |
| CAGAGACTGAACGGCAATCTTTCCACACTGTGGAACAATGCATTTGTGCCTAAACT TCTAGAGC |
a
Sense sequence; F, forward primer; R, reverse primer; WT, wild-type; MUT, mutant; MEF2C, myocyte enhancer factor 2C; TCF4, transcription factor 4.