TABLE I.

Test Agents and Associated Information

Test agent	CAS. No.	Vehicle	Treatment schedule	Dose level (mg/kg/day)	Rationale for top dose level
Aristolochic acids I/II	313–67–7	PBS	3 days × 1 cycle 3 days × 3 cycles	0, 5.5, 11, 22 0, 5.5, 11, 22	From literature, Bhalli et al. (2013)
Benzo[a]pyrene	50–32-8	Sesame oil	3 days × 1 cycle 3 days × 3 cycles	0, 62.5, 125, 250 0, 62.5, 125, 250	From literature, Phonethepswath et al. (2010)
Cisplatin	15663–27-1	0.9% Saline	3 days × 1 cycle 3 days × 3 cycles	0, 0.5, 1, 2 0, 0.5, 1, 2	Preliminary dose range finding study
Cyclophosphamide monohydrate	6055–19–2	Water	3 days × 1 cycle 3 days × 3 cycles	0, 3.75, 7.5, 15 0, 3.75, 7.5, 15	From literature, Dertinger et al. (2012)
Diethylnitrosamine	55–18–5	Water	3 days × 1 cycle 3 days × 3 cycles	0, 10, 20, 40 0, 10, 20, 40	From literature, Avlasevich et al. (2018)
1,2-Dimethylhydrazine-2HCl	306–37–6	Water	3 days × 1 cycle 3 days × 3 cycles	0, 7.5, 15, 30 0, 7.5, 15, 30	Preliminary dose range finding study
Dimethylnitrosamine	62–75–9	0.5% Methyl cellulose	3 days × 1 cycle 3 days × 3 cycles	0, 2.5, 5, 10 0, 2.5, 5, 10*	Preliminary dose range finding study
2,6-Dinitrotoluene	606–20–2	Sesame oil	3 days × 1 cycle 3 days × 3 cycles	0, 25, 50, 100 0, 25, 50, 100	Preliminary dose range finding study
Hydroxyurea	127–07–1	Water	3 days × 1 cycle 3 days × 3 cycles	0, 31.25, 62.5, 125 0, 31.25, 62.5, 125	From literature, Dertinger et al. (2012)
Melphalan	148–82–3	Sesame oil	3 days × 1 cycle 3 days × 3 cycles	0, 0.375, 0.75, 1.5 0, 0.75, 1.5, 3	From literature, Dertinger et al. (2012)
Temozolomide	85622–93–1	0.5% Methyl cellulose	3 days × 1 cycle 3 days × 3 cycles	0, 3.75, 7.5, 15 0, 3.75, 7.5, 15	Preliminary dose range finding study
Quinoline	91–22–5	Sesame oil	3 days × 1 cycle 3 days × 3 cycles	0, 75, 100, 125 0, 75, 100, 125	From literature, Avlasevich et al. (2018)
Vinblastine sulfate	143–67–9	0.9% Saline	3 days × 1 cycle 3 days × 3 cycles	0, 0.0625, 0.125, 0.25 0, 0.075, 0.125, 0.175	Preliminary dose range finding study