TABLE II.
Influence of Rat Dosing Schedule on Maximum Mean Pig-a Frequency
| Chemical | Dosing protocol | Harvest day | Max. daily dose (mg/kg/day); Total exposure (mg/kg) | Max. Mean RETCD59 −(×10−6)a | Max. Mean RBCCD59 −(×10−6) |
Study No., Citation |
|---|---|---|---|---|---|---|
| Benzo[a]pyrene | 3 day × 1 cycle | 7 | 250; 750 | 6.2 | 0.8 | LR180102, this article |
| 3 day × 3 cycles | 21 | 250; 2250 | 45.7 | 21.6 | LR180123, this article | |
| 28 day | 29 | 125; 3500 | 112.2 | 35.6 | PR111011, Torous et al. (2012) | |
| Cisplatin | 3 day × 1 cycle | 7 | 2; 6 | 1.6 | 0.5 | LR180529, this article |
| 3 day × 3 cycles | 21 | 2; 18 | 11.8 | 6.0 | LR180612, this article | |
| 28 day | 29 | 0.4; 11.2 | 26.3 | 6.2 | PR130702, Dertinger et al. (2014a) | |
| Cyclophosphamide | 3 day × 1 cycle | 7 | 15; 45 | 0.7 | 0.5 | LR180327, this article |
| 3 day × 3 cycles | 21 | 15; 135 | 7.4 | 2.3 | LR180206, this article | |
| 28 day | 29 | 5; 140 | 5.1 | 1.6 | PR110712, Dertinger et al. (2012) | |
| Diethylnitrosamine | 3 day × 1 cycle | 7 | 40; 120 | 0.6 | 0.5 | LR180306, this article |
| 3 day × 3 cycles | 21 | 40; 360 | 5.0 | 1.7 | LR180227, this article | |
| 28 day | 29 | 12.5; 350 | 4.2c | 1.0c | PR130910, Avlasevich et al. (2014) | |
| 28 day | 29 | 15; 420 | 3.9 | 1.1 | LR170717, Khanal et al. (2018) | |
| Melphalan | 3 day × 1 cycle | 7 | 1.5; 4.5 | 0.7 | 1.0 | LR180807, this article |
| 3 day × 3 cycles | 21 | 3; 27 | 10.0 | 4.1 | LR180904, this article | |
| 28 day | 29 | 0.75; 21 | 20.7 | 9.3 | PR110215, Dertinger et al. (2012) | |
| 28 day | 29 | 0.75; 21 | 11.1 | 4.4 | PR120904, Dertinger et al. (2014b) |
Abbreviations: RETCD59− = mutant phenotype reticulocytes; RBCCD59− = mutant phenotype erythrocytes.
a
As a point of reference, mean RETCD59−× 10−6 for vehicle control rats is generally within the range 0.5–1.5.
b
As a point of reference, mean RBCCD59−× 10−6 for vehicle control rats is generally within the range 0.5–1.5.
c
These group averages are based on n = 5 instead of 6; one severe outlier was removed.