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. 2021 Nov 4;12:737650. doi: 10.3389/fimmu.2021.737650

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Copyright © 2021 Hu, Zhang, Fan, Wang, Xu, Huang, Dai, Cao, Zhang, Liu and Cheng

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overview of the study design. First, we identified 24,556 candidate immune-related methylation probes. Then unsupervised cluster was used in a 683 TCGA glioma methylation cohort. A five-CpG-based prognostic signature was established and validated in five independent GEO datasets.