TABLE 1.
Fragment probes and control strains used for DNA hybridization experiments
| Pathotype | Target gene(s) | Fragment probes used (restriction endonuclease[s]) | Control strain(s)a | Reference(s) |
|---|---|---|---|---|
| EPEC | LEE A | pCVD453 (BglII/MluI) | E2348/69 (O127:H6) (LEE+bfpA+) | 16, 31 |
| LEE B | pCVD461 (EcoRI/SalI) | |||
| LEE C (eae) | pCVD443 (HindIII) | |||
| LEE D | pCVD460 (BglIII) | |||
| bfpA | pMSD207 (EcoRI) | |||
| EHEC | LEE A to D | As above | EDL933 (O157:H7) (LEE+hly+ stx1+ stx2+); EDL933cu (hly mutant) | 28, 31, 34 |
| hly | pCVD419 (HindIII) | |||
| stx1 | pNN110-18 (SmaI/PstI) | |||
| stx2 | pJN37-19 (BamHI) | |||
| STEC | stx1 and stx2 | pJN37-19 (BamHI) | EDL933 (O157:H7) | 34 |
| pNN110-18 (SmaI/PstI) | ||||
| ETEC | elt | pCVD403 (BamHI) | H10407 (LT+ STh+ STp+) | 17 |
| estp | pCVD426 (PstI) | |||
| esth | pCVD427 (EcoRI) | |||
| EIEC | Invasion plasmid | pSF55 (HindIII) | EI.37 | 20 |
| EAEC | Aggregative adherence plasmid | pCVD432 (EcoRI/PstI) | 042 (O44:H18) | 9 |
| DAEC | daaC | pSLM852 (PstI) | C1845 | 11 |
| CDEC | hlyAB, pap, cnf | pANN215 (HindIII) | 536, A70.1 (hly+ pap+ cnf+); 536-21 (hly− pap− cnf− mutant) | 19 |
| pRHU845 (HindIII) | ||||
| pSE266 (Sau3AI) | ||||
| CLDTEC | cdtAB | pCVD448 (EcoRV) | E6468/62 (O86:H34) | 39, 43 |
| cdtAB | pCP2100 (AvaI/SalI) |