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. Author manuscript; available in PMC: 2022 Sep 10.
Published in final edited form as: J Control Release. 2021 Jul 16;337:179–192. doi: 10.1016/j.jconrel.2021.07.018

Figure 7. Dendrimer-glucose and -galactose conjugates alter cellular interactions.

Figure 7.

A) Blocking GLUT-1 receptors on BV2 murine microglia by STF-31 reduces dendrimer-glucose (D-GLU) cellular internalization but does not impact uptake of unmodified dendrimers (D-OH), validating the GLUT-1 uptake mechanism of D-GLU. ** p < 0.01D, n.s. p > 0.1. B) Dendrimer internalization in IL4 and resting microglia is significantly greater with D-GLU than D-OH, indicating improved cellular internalization. * p < 0.05, *** p < 0.001. C) D-GLU exhibits similar brain uptake in healthy brain tissue and in the contralateral hemisphere of tumor-bearing brains and significantly greater than D-OH, indicating increased blood brain barrier penetration. ** p < 0.01, n.s. p > 0.1. D) Dendrimer-galactose conjugates (D-GAL) exhibits significantly greater membrane association in GL261 murine glioma cells than D-OH. Blocking of galectins with α-lactose knocks membrane levels of D-GAL down to similar levels as D-OH, validating D-GAL interactions with galectin surface receptors. * p < 0.05, ** p < 0.01. E) Cellular uptake of dendrimers of D-GAL is not altered with α-lactose treatment, indicating that D-GAL interactions with galectin receptors does not impact cellular internalization. n.s. p > 0.1.