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. 2021 Nov 18;18:223. doi: 10.1186/s12985-021-01695-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Flow cytometric analysis of EGFP fluorescing B-cells following rAAV transduction. a–c Bar graphs demonstrating the percentage of EGFP-expressing cells (y-axis) following transduction of different rAAVs (x-axis) as detected by flow cytometry in a uninfected primary B-cells, b primary B-cells 48 h after EBV infection, and c B-cells stably immortalized by the prototype EBV B95-8 virus, d–f Bar graphs demonstrate the percentage of EGFP-expressing cells (y-axis) detected in three different EBV + Burkitt lymphoma cell lines: d Raji, e P3HR1, and f Daudi as assessed by flow cytometry 48 h after transduction of different rAAV serotypes (x-axis). The EBV genomes of the respective tumor lines vary based on mutation, deletion and inversions. The bar graphs display mean ± SD, n = 3 independent experiments. A rank order of the serotypes producing the highest percentage of fluorescent cells is displayed under each histogram