FIG. 6.

Subcellular colocalization of Ad12 E1B 55-kDa protein and p53. G401-CC3 cells that constitutively express Ad12 E1B 55-kDa protein were grown on glass coverslip and fixed. Antibody staining of the cells was done as described previously (32). (A) p53 was visualized with anti-p53 antibody DO-1 and fluorescein-conjugated anti-mouse IgG antibody. (B) The Ad12 E1B 55-kDa protein was stained with anti-Ad12 E1B antibody anti-E1B and visualized with Texas red-conjugated anti-rabbit IgG antibody. (C) Nuclear staining with 4′,6′-diamidino-2-phenylindole (DAPI). (D) Merged image of panels A, B, and C. Colocalization of p53 and Ad12 E1B 55-kDa protein in a cytoplasmic body is indicated by an arrowhead.