Fig. 1.

Identification of host proteins associated with JEV 3′UTR. a A biotin-labeled JEV 3′UTR was incubated with BHK-21 cell lysates. Streptavidin was then applied to pull down the labeled RNA and associated host proteins. The eluates were subsequently subjected to SDS-PAGE analysis. Lane 1: protein size marker; lane 2: input of BHK-21 cell lysates; lane 3: BHK-21 cell lysates incubated with the non-biotinylated JEV 3′UTR; lane 4: BHK-21 cell lysates incubated with the biotinylated JEV 3′UTR. b Western blotting was performed, and both anti-FUBP3 and anti-FUBP1 antibodies were used to detect the presence of these two proteins