Fig. 2.

Knockdown of FUBP3 reduces JEV infectivity. a BHK-21 cells were transfected with siFUBP3 (5 µM) or si-scramble RNA (negative control), followed by culturing the cells for 24 h prior to the MTT assay. The si-scramble RNA-transfected cells were used as 100% of cell viability. The results shown here were the representative of three independent experiments. b BHK-21 cell lysates were harvested and protein expression was analyzed by Western blotting using anti-FUBP3 specific antibody. c The si-FUBP3-transfected-BHK-21 cells were then infected with JEV (MOI = 2) for 24 and 48 h, followed by analysis of viral titers using plaque forming assay (*p < 0.05; **p < 0.01). d To overexpress the FUBP3, pCMV-8 vector (control) and pCMV-FUBP3-Flag vector (8 µg) were transfected into BHK-21 cells for 24 h. Protein expression was analyzed by Western blotting as described above. e FUBP3-overexpressed BHK-21 cells were then infected with JEV (MOI = 2) for 24 and 48 h, followed by analysis of viral titers using plaque forming assay (*p < 0.05; **p < 0.01)