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. 2021 Nov 18;18:224. doi: 10.1186/s12985-021-01697-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Overexpression of FUBP3 colocalized with viral NS5 protein in JEV-infected BHK-21 cells. Flag-tagged FUBP3 protein that was expressed in BHK-21 cells 24 h prior to JEV infection. The mock- and JEV-infected BHK-21 cells were harvest at 48 h post-infection and co-immunostained with anti-NS5 (red) and anti-FUBP3 (green) antibodies. Subcellular localization of FUBP3 (panel b, f) and viral NS5 protein (panel g) in mock- and JEV-infected cells. The nucleus was stained with DAPI as shown in the merged images (panel a/d, e/h). Arrows showed the colocalization of FUBP3 with NS protein (panel e) in the cytoplasm of JEV-infected cells. The Pearson correlation coefficient (PCC) of images of FITC-FUBP3 and Texas Red-NS5 is 0.9946. Images are representative of three independent experiments that used three independent infections