Fig. 6.

FUBP3 relocated to the JE replication complex. BHK-21 cells were first transfected with the flag-tagged FUBP3 expressing plasmid to overexpress the FUBP3 protein and then proceeded to JEV infection. The mock-and JEV-infected BHK-21 cells were harvest at 48 h post-infection and co-immunostained with anti-dsRNA (red) and anti-FUBP3 (green) antibodies. Panel a–d are mock groups. Panel e–h and i–l are JEV infection groups (Images were taken from three independent experiments, and two representative groups were selected). Positive stained for dsRNA was only observed in the JEV-infected cells (panel h, i). The nucleus was stained with DAPI as shown in the images (panel a, e, f). The colocalization of FUBP3 with viral dsRNA protein (panel f, j) was detected in the cytoplasm of JEV-infected cells. The Pearson correlation coefficient (PCC) of images of FITC-FUBP3 and Texas Red-dsRNA is 0.9445. The Images are representative of three independent experiments that used three independent infections