Table 2.
Experimental design of 212 included studies
| Characteristics | N = 212 |
|---|---|
| Base medium | |
| DMEM | 141 (66.5%) |
| Customised media | 23 (10.8%) |
| Eagle minimal essential medium | 18 (8.5%) |
| DMEM/F12 | 11 (5.2%) |
| Neurobasal | 2 (0.9%) |
| Unspecified | 17 (8.0%) |
| Serum supplementation | |
| Yes | 190 (89.6%) |
| No | 1 (0.4%) |
| Unknown | 21 (9.9%) |
| Carbon dioxide (%) | |
| 5 | 156 (73.6%) |
| 6 | 1 (0.5%) |
| 10 | 1 (0.5%) |
| Unknown | 54 (25.5%) |
| Temperature | |
| 37°C | 160 (75.5%) |
| Unknown | 52 (24.5%) |
| Hypoxic condition | |
| Used | 5 (2.4%) |
| Unknown | 54 (25.5%) |
| Other methodological reporting | |
| Cell density | 154 (72.6%) |
| Number of cell passage | 16 (7.5%) |
| Number of replicates | 58 (27.4%) |
| Number of plate replicates | 42 (19.8%) |
DMEM Dulbecco’s Modified Eagle Medium, DMEM/F12 Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12