Fig. 2.

LncRNA Snhg6 reduces the stability of EZH2 protein. a The cellular localization of lncRNA Snhg6 was detected by Cy3-labeled lncRNA Snhg6 probe. Cy3-labeled 18S probe was used to indicate plasmid localization and Cy3-labeled U6 probe was used to indicate nuclear localization. DAPI was used to evaluate the cell nucleus. b Subcellular fractionation was isolated of MDSCs, and lncRNA Snhg6 localization was examined by qRT-PCR. 18S and U6 were used as cytoplasmic and nuclear indicators, respectively. c, d qRT-PCR were used to detect the expression of EZH2 at mRNA level. e, f Western blot were used to detect the expression of EZH2 at protein level. g Western blot were used to detect the expression of EZH2 with CHX (40 µg/ml) treated 0 h, 3 h and 6 h after transfecting si-Snhg6. h The statistical graph corresponding to the left. i RIP assays were used to investigate the ubiquitination of EZH2. Each expression had three replicates, ns: no significance; *p < 0.05; **p < 0.01