FIG. 2.

Elimination of inhibitory effects on PCR of serum from a hemodialysis patient by the automated specific capture with probes and magnetic B-F separation. HCV RNA-positive serum was obtained from a hemodialysis patient, who was anticoagulated with 3,400 U of heparin, at 0, 1, 3, and 5 h following hemodialysis. Serum samples were diluted 1:10 in HCV RNA-negative serum. RNA was extracted from undiluted (A) and diluted (B) aliquots by the manual method (the SepaGene RV-R method) and then amplified for HCV RNA and the internal control with the COBAS AMPLICOR system. HCV RNA was extracted from undiluted aliquots by the automated extraction method based on probe capture and magnetic B-F separation, and then amplified for HCV RNA with the COBAS AMPLICOR system (C). Data are means of duplicate assays. ●, HCV RNA; ▴, internal control.