Fig. 5.

Dysregulation of DNA damage response-associated proteins and the HIF-1α/miR-210/Rad52 signaling pathway in BEAS-2B cells after long-term Nano-Ni exposure. BEAS-2B cells were treated with 0, 0.25 and 0.5 µg/mL of Nano-Ni for 21 cycles as described in the Methods. A is the result of a single Western blot experiment. Nuclear protein was subjected to Western blot. Equal nuclear protein loading was verified by Coomassie Brilliant Blue staining. B, C are quantified band densitometry readings averaged from 3 independent experiments ± SEM of Western blot results. D miR-210 expression was determined by real-time PCR. Values of miR-210 expression was normalized to the endogenous control U6 snRNA. Data are shown as mean ± SEM (n = 3). *, p < 0.05 vs. control