Skip to main content
. Author manuscript; available in PMC: 2021 Nov 18.
Published in final edited form as: Cell Rep. 2021 Nov 2;37(5):109910. doi: 10.1016/j.celrep.2021.109910

Figure 5. RBFOX2 binding sites near dPASs are critical for APA regulation of the Slc25a4 gene.

Figure 5.

(A) Cartoon representation of luciferase-Slc25a4 poly(A) reporter constructs that harbor both pPASs and dPASs of Slc25a4 in the 3′UTR with or without mutated RBFOX2 binding motifs (underlined) located downstream of the dPAS.

(B) qRT-PCR analysis of dPAS usage when normalized to total mRNA levels in HEK293 cells expressing WT or RBFOX2 binding site mutant poly(A) luciferase-Slc25a4 3′UTR constructs. n = 24 for WT, n = 13 for mutant samples from three independent experiments, ****p < 0.0001.

(C) Relative firefly luciferase levels in HEK293 cells expressing WT or RBFOX2 binding site mutant poly(A) luciferase-Slc25a4 3′UTR constructs. n = 12 for WT samples, n = 14 for mutant samples from three independent experiments, ****p < 0.0001.