KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| RBFOX2 | Abcam | Cat# ab57154; RRID:AB_2285090 |
| TPM1 | Cell Signaling | Cat# 3910; RRID:AB_2205654 |
| α-tubulin | Sigma-Aldrich | Cat# T6074; RRID:AB_477582 |
| mitofusin | Abcam | Cat# 57602; RRID:AB_2142624 |
| OPA1 | Abcam | Cat# 157457; RRID:AB_2864313 |
| COX4 | Abcam | Cat# 110272; RRID:AB_10862891 |
| CYTB | Santa Cruz | Cat# sc-11436; RRID:AB_2088887 |
| ANT1:Slc25a4 | Abcam | Cat# ab102032; RRID:AB_10710263 |
| Critical commercial assays | ||
| Dual Luciferase Reporter Assay System | Promega USA | Cat# E1980 |
| Deposited data | ||
| Raw and deposited data (PAC seq Nanopore seq) | NCBI SRA | PRJNA517125 |
| Experimental models: Cell lines | ||
| H9c2 myoblasts (Rattus Norvegicus, obtained from embryonic rat heart tissue) | ATCC | Cat# CRL-1446 |
| HEK293 (Homo sapiens, female embryonic kidney cells) | ATCC | Cat# ACS-4500 |
| Oligonucleotides | ||
| Primers (See Table S2) | This paper | N/A |
| scrambled siRNA | Thermofisher Scientific | Cat# AM4611 |
| Rbfox2 siRNA | Thermofisher Scientific | Cat# s96620 |
| Recombinant DNA | ||
| human GFP-RBFOX2 (transcript variant 3) | pEGFP rbFOX2 was a gift from Nicolas Charlet-Berguerand | Addgene plasmid # 63086 ; http://addgene.org/63086 |
| Flag-ANT1 | SinoBiological | Cat# HG17969-NF |
| pcDNA 5 | Invitrogen | Cat# V6520-20 |
| Luciferease-Slc25a4 polyA reporter (see Star Methods) | This paper | N/A |
| Software and algorithms | ||
| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/index.html |
| Poly(A)CLICK-seq pipeline | Routh, 2019 | https://sourceforge.net/projects/DPAC-Seq |