Skip to main content
. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Mol Microbiol. 2021 Jun 25;116(2):707–722. doi: 10.1111/mmi.14765

Figure 3. Cells are more sensitive to yneA induction in the absence of the negative regulators ddcP, ctpA and ddcA.

Figure 3.

(A) Spot titer assay using B. subtilis strains WT (PY79), ΔddcP ΔctpA ΔddcA (PEB639), ΔyneA::loxP (PEB439), ΔddcP ΔctpA ΔddcA ΔyneA::loxP (PEB643), ΔddcP ΔctpA ΔddcA amyE::Phy-yneA (PEB844), ΔyneA::loxP amyE::Phy-yneA (EAM46) and ΔddcP ΔctpA ΔddcA ΔyneA::loxP amyE::Phy-yneA (EAM56) spotted on the indicated media. (B) Spot titer assay using B. subtilis strains WT (PY79), ΔddcP ΔctpA ΔddcA (PEB639), ΔyneA::loxP (PEB439), ΔddcP ΔctpA ΔddcA ΔyneA::loxP (PEB643), ΔddcP ΔctpA ΔddcA amyE::Phy-yneA (PEB844), ΔyneA::loxP amyE::Phy-yneA (EAM46) and ΔddcP ΔctpA ΔddcA ΔyneA::loxP amyE::Phy-yneA (EAM56) spotted on the indicated media.