Table 1.
Mean peak height (PH) of significantly different lipids in X-deletion sample injections (n = 3) compared to control sample injections (n = 3) and their ratio in positive mode (ID methods: 1 = More than one MS/MS fragment m/z match to in silico database from data dependent (top 5), 2 = all-ion fragmentation MS/MS fragment m/z match, 3 = Only one fragment m/z match).
| Lipid Name | m/z | Ion/Adduct | ID methods | Mass Accuracy (ppm) | Average X-deletion PH (n = 3) | X-deletion RSD (%) | Average Control PH (n = 3) | Control RSD (%) | X-deletion/ Control |
|---|---|---|---|---|---|---|---|---|---|
| PC(38:6) | 792.5903 | [M+H]+ | 3 | −0.5 | 6.40E+05 | 3.47 | 1.27E+04 | 52.65 | 50.5 |
| PE(40:8) | 788.5264 | [M+H]+ | 3 | 4.3 | 2.37E+05 | 4.33 | 9.71E+03 | 26.77 | 24.4 |
| Plasmenyl-PC(P-16:0/20:4) | 766.5755 | [M+H]+ | 1,2 | 1.3 | 1.28E+08 | 20.3 | 5.97E+06 | 2.81 | 21.4 |
| PC(38:7) | 790.5743 | [M+H]+ | 3 | −1 | 4.63E+06 | 2.98 | 2.83E+05 | 12.88 | 16.3 |
| BMP(18:2_20:3) | 814.5615 | [M+H]+ | 2 | 2.8 | 2.97E+05 | 0.84 | 1.83E+04 | 23.14 | 16.2 |
| HexCer-NS(d18:1/17:0) | 714.5894 | [M+H]+ | 1 | 2.2 | 5.57E+05 | 10.51 | 3.72E+04 | 18.16 | 15 |
| Plasmenyl-PC(P-18:1/20:4) | 792.5911 | [M+H]+ | 1 | 0.3 | 7.55E+07 | 6.85 | 5.19E+06 | 6.87 | 14.5 |
| PC(40:7) | 818.6067 | [M+H]+ | 3 | 0.4 | 8.62E+05 | 9.59 | 6.03E+04 | 18.22 | 14.3 |
| LPC(18:1) | 506.3627 | [M+H]+ | 3 | −3.2 | 5.55E+05 | 10.28 | 5.02E+04 | 9.94 | 11.1 |
| BMP(18:2_22:4) | 840.577 | [M+H]+ | 1,2 | 2.5 | 5.14E+06 | 2.84 | 4.86E+05 | 2.91 | 10.6 |
| Plasmenyl-PC(P-16:0/22:4) | 794.6061 | [M+H]+ | 1 | 0.4 | 3.28E+07 | 5.28 | 3.40E+06 | 6.69 | 9.65 |
| Plasmenyl-PC(P-18:0/20:4) | 794.6055 | [M+H]+ | 1,2 | −0.3 | 2.46E+07 | 5.91 | 2.58E+06 | 4.07 | 9.55 |
| BMP(18:2_20:4) | 812.5456 | [M+H]+ | 1 | 2.5 | 5.43E+05 | 0.19 | 5.71E+04 | 3.04 | 9.5 |
| PC(36:5) | 780.5921 | [M+H]+ | 3 | −2.8 | 1.63E+06 | 44.43 | 1.78E+05 | 4.85 | 9.2 |
| PC(34:5) | 752.5256 | [M+H]+ | 3 | 3.4 | 5.26E+05 | 7.02 | 6.21E+04 | 32.34 | 8.5 |
| TG(18:1_18:1_20:4) | 924.804 | [M+NH4]+ | 1 | 2.8 | 1.23E+06 | 3.86 | 1.47E+05 | 0.44 | 8.4 |
| PC(38:4) | 796.622 | [M+H]+ | 3 | 0 | 9.43E+06 | 1.46 | 1.15E+06 | 4.52 | 8.2 |
| PC(40:6) | 820.6216 | [M+H]+ | 3 | −0.5 | 3.85E+06 | 4.3 | 4.87E+05 | 3.29 | 7.9 |
| Plasmenyl-PC(P-18:1/22:4) | 820.6225 | [M+H]+ | 1 | 1.3 | 1.93E+07 | 4.5 | 2.54E+06 | 5.5 | 7.6 |