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. Author manuscript; available in PMC: 2021 Nov 18.
Published in final edited form as: Nat Med. 2021 Jul 9;27(9):1600–1606. doi: 10.1038/s41591-021-01443-1

Extended Data Fig. 6 ∣. sc-RNA-seq shows increased neuronal population in PCCOs.

Extended Data Fig. 6 ∣

a, Split UMAPs show BCL11B (CTIP2) expression in COs and PCCOs. b, Number of the BCL11B+ cells increased in PCCO compared to CO. Number of cells in circle shown in a were counted. N/T represents the percentage of BCL11B+ cells (N) in total cells (T). c, Split UMAPs show SATB2 expression in COs and PCCOs. d, Number of the SATB2+ cells increased in PCCOs compared to COs. Number of cells in circle shown in c were counted. N/T represents the percentage of SATB2+ cells (N) in total cells (T). e, Both deeper cortical layers and upper cortical layers markers were increased in PCCOs compared to COs. TBR1 and BCL11B represent deeper cortical layers, and CUX1 and SATB2 label the upper cortical layers. COs and PCCOs from two independent hiPSC lines were harvested at div74 for RNA extraction followed qPCR. GAPDH used as reference. n = 12 includes 3 independent biological replicates (including COs and PCCOs from individual hPSC lines, replicates from different lines shown in different colors) and 4 technical replicates for each of the biological replicate.

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