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. 2021 Nov 15;9:e12290. doi: 10.7717/peerj.12290

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© 2021 Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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RdRP protein sequences were aligned and phylogenetic trees constructed by IQ-TREE using maximum likelihood with 10,000 bootstrap cycles. The final trees were drawn using FigTree v1.42. Asterisks indicate diatom viruses. Viruses identified in this study are indicated by boxes. Virus abbreviations (genome accession numbers): ADV: Acheta domesticus virus (DQ112164.1); ABPV: Acute bee paralysis virus (NC_002548.1); AGV1: Aphis glycines virus 1 (MK533146.1); APV: Acyrthosiphon pisum virus (NC_003780.1); APLV1: Antarctic picorna-like virus 1(NC_030232.1); APLV2: Antarctic picorna-like virus 2 (NC_030233.1); APLV3: Antarctic picorna-like virus 3 (NC_030234.1); APLV4: Antarctic picorna-like virus 4 (NC_030235.1); APIV: Antheraea pernyi iflavirus (NC_023483.1); ALPV: Aphid lethal paralysis virus (NC_004365.1); AGRNAV: Asterionellopsis glacialis RNA virus (NC_024489.1); AssRNAV01: Aurantiochytrium single-stranded RNA virus 01 (NC_007522.1); BBV: Brevicoryne brassicae virus (NC_009530.1); BDV: Bat dicistrovirus (MH370347.1); BQCV: Black queen cell virus (NC_003784.1); BMIV: Bombyx mori iflavirus (LC214356.1); ChspRNAV2: Chaetoceros sp. RNA virus 2 (AB639040.1); ChSfrRNAV01: Chaetoceros socialis f. radians RNA virus 01 (NC_012212.1); ChTRNAV01: Chaetoceros tenuissimus RNA virus 01 (NC_038321.1); ChTRNAVT-II: Chaetoceros tenuissimus RNA virus type-II (NC_025889.1); CrPV: Cricket paralysis virus (NC_003924.1); DWV: Deformed wing virus (NC_004830.2); DCPLV: Diaphorina citri picorna-like virus (KT698837.1); DCPV: Dinocampus coccinellae paralysis virus (NC_025835.1); DCV: Drosophila C virus (NC_001834.1); DVvV1: Diabrotica virgifera virgifera virus 1 (KY064174.1); EOPLV: Ectropis obliqua picorna-like virus (NC_005092.1); GDV: Goose dicistrovirus (NC_029052.1); GNV1: Graminella nigrifrons virus 1 (NC_026733.1); FEV1: Formica exsecta virus 1(MH133342.1); FEV2: Formica exsecta virus 2 (NC_023022.1); FSPLV: Fur seal picorna-like virus (NC_035110.1); HaARNAV: Halastavi arva RNA virus (NC_016418.1); HHV: Halyomorpha halys virus (NC_022611.1); HEIV: Heliconius erato iflavirus (NC_024016.1); HeARNAV: Heterosigma akashiwo RNA virus (NC_005281.1); HiPV: Himetobi P virus (NC_003782.1); HCV1: Homalodisca coagulata virus-1 (NC_008029.1); IFV: Infectious flacherie virus (NC_003781.1); IAPV: Israeli acute paralysis virus (NC_009025.1); KaV: Kakugo virus (AB070959.1); KBV: Kashmir bee virus (NC_004807.1); KFV: Kelp fly virus (NC_007619.1); LSHV1: Laodelphax striatella honeydew virus 1 (NC_023627.1); LSPLV2: Laodelphax striatellus picorna-like virus 2 (NC_025788.1); LLV1: Lygus lineolaris virus 1 (NC_038301.1); LDIV1: Lymantria dispar iflavirus 1 (NC_024497.1); MCDV: Mud crab dicistrovirus (NC_014793.1); MRNAVBC-2: Marine RNA virus BC-2 (NC_043542.1); MRNAVBC-3: Marine RNA virus BC-3 (NC_043543.1); MRNAVJP-A: Marine RNA virus JP-A (NC_009757.1); MRNAVJP-B: Marine RNA virus JP-B (NC_009758.1); MRNAPAL156: Marine RNA virus PAL156 (NC_029307.1); MRNAVSF-1: Marine RNA virus SF-1 (NC_043515.1); MRNAVSF-2: Marine RNA virus SF-2 (NC_043518.1); MRNAVSF-3: Marine RNA virus SF-3 (NC_043519.1); MRTV: Macrobrachium rosenbergii Taihu virus (NC_018570.2); NeDVTFN-2012: Nedicistrovirus TFN-2012 (JQ898341.1); NLCV: Nilaparvata lugens C virus (KM270560.1); NLHV1: Nilaparvata lugens honeydew virus 1 (NC_038302.1); NLHV2: Nilaparvata lugens honeydew virus 2 (NC_021566.1); NLHV3: Nilaparvata lugens honeydew virus 3 (NC_021567.1); NV: Nora virus (NC_007919.3); OIIV1: Opsiphanes invirae iflavirus 1(NC_027917.1); PNV: Perina nuda virus (NC_003113.1); PSIV: Plautia stali intestine virus (NC_003779.1); RhSRNAV01: Rhizosolenia setigera RNA virus 01 (NC_018613.1); RhPV: Rhopalosiphum padi virus (NC_001874.1); SaV: Sacbrood virus (NC_002066.1); SssRNAV: SBPV: Slow bee paralysis virus (NC_014137.1); SIV1: Solenopsis invicta virus 1 (NC_006559.1); SEIV1: Spodoptera exigua iflavirus 1 (NC_016405.1); SEIV2: Spodoptera exigua iflavirus 2 (NC_023676.1); TSV: Taura syndrome virus (NC_003005.1); ThPIV1: Thaumetopoea pityocampa iflavirus 1 (NC_026250.1); TMV: Tomato matilda virus (MK517476.1); TrV: Triatoma virus (NC_003783.1); VVARLV: Vespa velutina associated triato-like virus (GGL51738.1). The sequences of KB2009con15, KB2009con28, KB2009con55, KB2009con74, KB2009con88 and KB2010con16 (Culley et al., 2014) were kindly provided by Dr. Alexander I. Culley, Department de Biochimie, de Microbiologie et de Bio-informatique, Universite Laval, Quebec City, Quebec, Canada.