FIG. 9.
Mdm2 and p21WAF1/CIP1 RNA expression in tsBN462 and BHK21 cells. tsBN462 and BHK cells were split and incubated 24 h at 32°C. They were then (zero time point) transferred to 39°C and incubated in the absence or presence of mitomycin C (5 μg/ml) or kept at 32°C and treated with mitomycin C. At the indicated time points (0, 0.5, 1, 2, 4, 8, and 10 h), samples were processed for Northern blotting, hybridized with 32P-labelled Mdm2, p21WAF1/CIP1, and 36B4 (loading control) probes, and quantified by PhosphorImager analysis. The results are presented as fold stimulation relative to the zero time point. One representative experiment out of six is shown.