Figure 4:
KLF4 requires the STAT3-binding domain to induce mature spine formation. (A) Schematic presentation of different KLF4 deletion constructs. (B) DIV5–6 hippocampal neurons were transfected with Clover-βactin along with wtKLF4, KLF4-N400, KLF4-N454, or KLF4-N454. The neurons were fixed, and their spine densities were analyzed on DIV12. All data were analyzed with Kruskal-Wallis test followed by Dunn’s multiple comparison. Data were represented as mean ± SEM (***p<0.001, **p<0.01, *p<0.05).