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. Author manuscript; available in PMC: 2021 Nov 18.
Published in final edited form as: Mol Cell Neurosci. 2020 May 11;106:103500. doi: 10.1016/j.mcn.2020.103500

Figure 6:

Figure 6:

Both KLF4 and SOCS3 decrease STAT3 activity and inhibition of STAT3 increases spinogenesis. (A, B) DIV5–6 hippocampal neurons were transfected with pCAGGS-Clover and either wtKLF4, shKLF4, SOCS3, or shSOCS3. After 2 hours of leptin treatment on DIV7, neurons were fixed and stained for pSTAT3. (C,D) Hippocampal neurons were transfected on DIV5–6 with Clover-βactin ± caSTAT3, followed by leptin stimulation on DIV7. Neurons were fixed and analyzed on DIV12. These data were analyzed with Kruskal-Wallis test followed by Dunn’s multiple comparison. (E) HEK293T cells were transfected with STAT3-myc and ±shSTAT3 construct. Western blotting was done with anti-c-myc and anti-β-actin antibodies to show the efficiency of knockdown. (F) DIV5–6 hippocampal neurons were transfected with Clover-βactin and either shSTAT3, scrambled shRNA, or dnSTAT3, followed by leptin stimulation on DIV7. Neurons were fixed and analyzed on DIV12. All data were analyzed with Kruskal-Wallis test followed by Dunn’s multiple comparison. Data were represented as mean ± SEM (***p<0.001, **p<0.01, *p<0.05).