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. Author manuscript; available in PMC: 2022 Oct 1.

Published in final edited form as: J Mol Cell Cardiol. 2021 Jun 15;159:62–79. doi: 10.1016/j.yjmcc.2021.06.004

Figure 1. — C57BL/6J mice were subjected to sham procedures or LAD ligation. Immunohistochemistry was performed on cryosections prepared from MI hearts. Immunoblot analysis was performed using total protein extracted from cMPs isolated from sham hearts or MI tissues. A. Tissue sections from hearts three days after LAD ligation were labeled with antibodies made against p-S6 and monocyte/macrophage marker CD68. Images were taken from both the infarct and remote regions. n=3 hearts. B. Tissue sections from the same MI cohorts as in A were labeled with neutrophil and p-S6 antibodies. C. Quantification of p-S6+ and CD68+ areas relative to the total area using ImageJ. The percentage of p-S6+CD68+ area to the total p-S6+ area was plotted on the right axis. n=3 hearts. D. Immunoblot analysis of p-P70S6K and p-S6 using cMPs isolated from MI tissue 3-day and 7-day after LAD ligation. E. Quantification of p-P70S6K and p-S6 from different experiments performed as in D. Each data point in sham represents 3 to 4 hearts combined, total 10 to 12 hearts used. In the infarct cohorts, some data points are from pooled infarct tissue. Total n= 6 to 9 hearts at each time point. * p<0.05 ** p<0.01.

Figure 1. — C57BL/6J mice were subjected to sham procedures or LAD ligation. Immunohistochemistry was performed on cryosections prepared from MI hearts. Immunoblot analysis was performed using total protein extracted from cMPs isolated from sham hearts or MI tissues. A. Tissue sections from hearts three days after LAD ligation were labeled with antibodies made against p-S6 and monocyte/macrophage marker CD68. Images were taken from both the infarct and remote regions. n=3 hearts. B. Tissue sections from the same MI cohorts as in A were labeled with neutrophil and p-S6 antibodies. C. Quantification of p-S6+ and CD68+ areas relative to the total area using ImageJ. The percentage of p-S6+CD68+ area to the total p-S6+ area was plotted on the right axis. n=3 hearts. D. Immunoblot analysis of p-P70S6K and p-S6 using cMPs isolated from MI tissue 3-day and 7-day after LAD ligation. E. Quantification of p-P70S6K and p-S6 from different experiments performed as in D. Each data point in sham represents 3 to 4 hearts combined, total 10 to 12 hearts used. In the infarct cohorts, some data points are from pooled infarct tissue. Total n= 6 to 9 hearts at each time point. * p<0.05 ** p<0.01.